Irf reporter assay
WebTumor heterogeneity dominates tumor biological behavior and shapes the tumor microenvironment. However, the mechanisms of tumor genetic features modul… WebAug 1, 2009 · IRF4 directly binds the Il10 gene as evidenced by chromatin immunoprecipitation assay, and regulates Il10 control elements in a reporter assay. IRF4 …
Irf reporter assay
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WebReporter Gene Assays Reporter gene assays are invaluable for studying the regulation of gene expression by cis- or trans-acting factors, or for measuring activation of cell … WebFeb 5, 2024 · ACE2-(TMPRSS2) expressing cells HEK-Blue™ hACE2 Cells A549-hACE2 Cells A549-hACE2-TMPRSS2 Cells이 세포들은 세포 표면에서 높은 수준의 host receptor ACE2를 단독 혹은 host protease TMPRSS2와 결합하여 발현합니다. 따라서 이러한 세포는 SARS-CoV-2 Spike pseudotyped lentiviral particle에 의한 감염이 가능하며, 특히 A549 세포에 …
WebHCT116-Dual™ cells express a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the IL-12 p40 minimal promoter fused to five NF-κB and AP-1 binding sites. HCT116-Dual™ cells also … WebIRFs are required to submit quality data for the IRF Quality Reporting Program beginning on 10/01/2012. During the first year of the IRF Quality Reporting Program, IRFs are required …
WebOr IECs were infected or mock-infected with PEDV for 12 h prior to transfection the luciferase reporter plasmids alone or cotransfection the indicated expression plasmids (0.5 μg). The cell lysates were harvested and luciferase activity was analyzed using a dual-luciferase assay system and a luminometer (Turner BioSystems, Inc. Sunnyvale, CA ...
WebApr 12, 2024 · The global Reporter Gene Assay market size is projected to grow from USUSD 1344.7 million in 2024 to USUSD 2010.3 million in 2029; it is expected to grow at a CAGR of 5.9Percent from 2024 to 2029 ...
WebMar 29, 2024 · Dual-luciferase reporter assay Artificially synthesized SOX4 promoter sequence fragments were inserted into the pMIR-reporter (Promega) after enzyme digestion. Next, the correctly sequenced luciferase reporter plasmids were respectively cotransfected with LV-NC, LV-LIN00470, LV-EGR2, si-NC, and si-LINC00470 into U87 cells. how to reset break glassWebThe Interferon Regulatory Factor (IRF) reporter (Luc)-THP-1 cell line is designed to study the activation and signaling of Cytosolic DNA Sensors (CDS) in the human monocytic cell line … The One-Step™ Luciferase Assay System is designed for high-throughput, sensitive … north carolina race car driversWebInvivoGen offers several engineered THP-1 cell lines: THP1 NF-κB cells were specifically designed for monitoring the NF-κB signal transduction pathway in a physiologically … how to reset brightsign playerWebTranscription factors of the interferon regulatory factor (IRF) family have been identified as critical mediators of early inflammatory gene transcription in infected cells. We recently determined that, besides IRF-3 and IRF-7, IRF-5 serves as a direct transducer of virus-mediated signaling. north carolina radio stationsWebInvivoGen reporter cells are designed to monitor the activation of intracellular signaling pathways. These engineered cell lines are used for cell-based assays of cytokine, PRR, agonist or antagonists. These cells stably express … how to reset britaWebThe ISRE Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. how to reset british gas smart meter monitorWebRather, PLpro interacts with IRF-3 and inhibits the phosphorylation and nuclear translocation of IRF-3, thereby disrupting the activation of type I IFN responses through either Toll-like receptor 3 or retinoic acid-inducible gene I/melanoma differentiation-associated gene 5 pathways. ... activation inhibit and luciferase reporter assay: 1, 2, 3 ... north carolina raffle law