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Thy-1 gfp jax

Webb1 okt. 2000 · Most sensory neurons were YFP-positive in thy1-YFP line G (a), about 10%–20% of neurons were labeled in thy1-GFP line I (b) and thy1-CFP line 4 (c), and only a few percent of neurons were labeled in thy1-GFP line O (d). In each case, whole DRGs from a lumbar level were mounted and imaged. Webb011070 B6;CBA-Tg(Thy1-EGFP)SJrs/NdivJ These thy1 -GFP-S transgenic mice show EGFP labeling in the superficial layers of the neocortex, and may be useful in neurobiological …

Besides neuro-imaging, the Thy1-YFP mouse could serve for

WebbCharacterization of a thy1.2 GFP transgenic mouse reveals a tissue-specific organization of the spinal dorsal horn Genesis. 2007 Nov;45(11):679-88. doi: 10.1002/dvg.20331. Authors Mino D Belle 1 , Edward F Pattison, Ornsiri Cheunsuang, Anika Stewart, Ina Kramer, Markus Sigrist, Silvia Arber, Richard Morris. Affiliation 1 Department of ... Webb12 aug. 2024 · When using the thy1-YFP-H mouse strain in a publication, please cite the originating article(s) and include JAX stock #003782 in your Materials and Methods … diversity the movie https://redhousechocs.com

Protocol 25728 - Tg(Thy1-YFP)HJrs-alternate2 - jax.org

WebbThe genotyping protocol (s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). To genotype animals, JAX … Webb15 sep. 2024 · Novel transgenic mouse lines facilitate discovery, but must be understood to be effective. The purpose of this study was to characterize the sensory and motor behavior of a common transgenic mouse line (Thy1-GFP-M) before and after SCI. Thy1-GFP-M positive (TG+) mice and their transgene negative littermates (TG-) were acquired … WebbInvitrogen Anti-CD90.1 (Thy-1.1) Monoclonal (HIS51), eBioscience™, Catalog # 14-0900-81. Tested in Immunohistochemistry (Frozen) (IHC (F ... . b Fluorescent microscopic image demonstrating fluorescence of MSCs labeled with GFP 2 weeks after implantation. c Flow cytometry analysis of surface antigens of MSCs (blue is the isotype, green ... cra class 36

003782 - thy1-YFP-H Strain Details - The Jackson …

Category:Retinal microvascular and neuronal pathologies probed in vivo by ...

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Thy-1 gfp jax

A new model for facial nerve research: the novel transgenic Thy1-GFP …

Webb27 okt. 2014 · Thy1 functions as a cell adhesion molecule and is involved in cell-cell and cell-extracellular matrix interactions. Its occurrence on many cell types suggests the possibility of different... Webb7 jan. 2024 · The scRNA-seq data analyses revealed that Thy-1.1 − GFP + Treg cells from Foxp3 LSL/y Cd4 creERT2 R26 Tom mice had equivalent and shared gene expression …

Thy-1 gfp jax

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Webb27 okt. 2014 · Thy1 functions as a cell adhesion molecule and is involved in cell-cell and cell-extracellular matrix interactions. Its occurrence on many cell types suggests the … WebbThy1.1-GFP was a gift from Britt Glaunsinger (Addgene plasmid # 131703 ; http://n2t.net/addgene:131703 ; RRID:Addgene_131703) For your References section: …

Webb27 dec. 2024 · Mutation details : The transgene contains a YFP gene under the direction of regulatory elements derived from the mouse Thy1 gene. The regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4, but lacking exon 3 and its flanking … WebbAnimal care and use. Commercially-available male C57BL/6J mice (WT) were purchased for experiments, while homozygous IL-4/GFP-enhanced transcript (IL-4-GFP) mice (JAX #004190), IL-4 KO mice (JAX #002253; on the C57BL6J background), and Thy1-GFP mice (JAX # 007788; on the C57BL6J background) were bred and used at 7 to 10 weeks old …

Webb007612 B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J These transgenic mice express the light-activated ion channel, Channelrhodopsin-2, fused to Yellow Fluorescent Protein under …

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Webb14 mars 2024 · Thy-1 EGFP M line (JAX stock number 007788) and Thy-1 YFP H line (JAX stock number 003782) transgenic mice, separately expressing GFP and YFP tag in the whole brain were used in this protocol. All of the mouse procedures were approved by the Institutional Animal Ethics Committee of the Huazhong University of Science and … cra class 14.1 assetsWebbConclusions: The Thy1-GFP rat is a novel transgenic tool that enables direct visualization of facial nerve regeneration after injury. The utility of this model extends to a variety of … diversity theoryWebb15 juli 2002 · GFP fluorescence ( x ‐axis) and Thy‐1.1 surface expression, detected by an APC‐labeled anti‐Thy‐1 antibody ( y ‐axis), were analyzed by two‐color flow cytometry. The numbers in the quadrants indicate the percentages of viable cells expressing pSV2Thy‐1.1 alone, pSVEd2G alone, or pSVEd2G and Thy‐1.1. cra class 55Webb18 okt. 2012 · At least one class of green fluorescent protein (GFP)-based GECIs, the GCaMP family, has been effective for detecting calcium dynamics induced by neuronal activity in multiple model organisms ( Muto et al., 2011; Reiff et al., 2005; Tian et al., 2009; Warp et al., 2012 ). crack 中文WebbRRID:IMSR_JAX:007788. Common Name: Thy1-GFP line M. These Thy1-GFP-M transgenic mice may be useful in neurobiological studies for fluorescent labeling of neural tissues, … cra class for appliancesWebb23 mars 2024 · 1:5,000 in sterile saline and then mixed in a 1:1 ratio with AAV2/1- CAG-Flex-EGFP (Addgene 51502-AA V1, 2.96 × 10 13 gc/ml). A total of 100 nl of the virus mixture was injected at a rate of cra class for cameraWebbProtocol 23870: Standard PCR Assay - Tg (Thy1-GFP) Version 1. 2 Notes The genotyping protocol (s) presented here have been optimized for reagents and conditions used by … cra classes for capital cost allowance